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Oil-Immersion
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Oil-Immersion

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Description

Oil immersion is a microscopy technique used to increase the resolution and magnification of microscope images, particularly when viewing specimens with high magnification objectives (typically 40x or higher). A drop of immersion oil, which has a refractive index close to that of glass, is placed on the specimen and the objective lens is then lowered to immerse in the oil. This reduces the refraction of light and increases numerical aperture, resulting in clearer and sharper images with improved detail. Oil immersion is commonly used in microbiology and histology for observing fine cellular structures and bacteria at high magnification.

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